GR | 建植过程中杂交和低地生态型柳枝稷选择的有力依据

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Table 1: The sampling effort and germplasm history for 46 half-sib families

Note: Families from the same germplasm source were collected from a common and isolated cross-pollination block. Samples indicate the number of DNA pools sequenced for each stage. Unequal sample numbers are due to the unbalanced experimental design and reduced sampling effort during Year 3.

Table 2: Candidate smoothing spline-defined windows for selection during sward establishment based on their occurrence as outliers in a minimum of three half-sib families based on the W-statistic

Note: Reported window positions are the narrowest band of markers which maximize the number of overlapping windows. Window Fst, W-statistics, and read depths are the mean values for all significant families within overlapping windows.

Figure 1: Reference allele frequencies of single-SNP outlier windows in DNA pools collected from half-sib families in a greenhouse (GH) and in the first year, the second year, or the third year of sward establishment. Families are derived from either the Liberty cultivar (a), or diverse Lowland populations (b). Each gray line indicates a family and includes all 26 families used in the analysis (18 within Liberty and 8 within Lowland). Black lines indicate the mean allele frequency of all families within the breeding population.

Figure 2: Predicted changes in genomically estimated breeding values (ΔGEBVs) during establishment in switchgrass seeded swards. (a) and (b) GEBVs of winter survivorship scores. (c) and (d) GEBVs of heading dates. Line colors indicate the initial (GH) GEBVs for each family, while the line trajectories indicate the change in GEBV over the course of sward establishment. GEBV, genomically estimated breeding value.

Figure 3: Principal components generated from marker data (m = 35 478) from the FLOMAS switchgrass trial containing four breeding groups. Point colors indicate genomically estimated breeding values for establishment survival based on training data from the hybrid breeding group. Units for sward survival were arbitrarily assigned scores (greenhouse samples = 0, Year 1 sward samples = 1, Year 2 sward samples = 2, Year 3 sward samples = 3).

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